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Gene Summary for ASCL2 Gene

Official Symbol ASCL2 Gene Type protein-coding
Full Name achaete-scute family bHLH transcription factor 2 Entrez 430
Ensembl ID ENSG00000183734 HGNC 739
Aliases ASH2, HASH2, MASH2, bHLHa45 Chromosome Location Chromosome 11, NC_000011.10 (2268498..2270588, complement)
Expression Broad expression in colon (RPKM 4.3), placenta (RPKM 2.5) and 14 other tissues
Summary This gene is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. Involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system. [provided by RefSeq, Jul 2008]

Paper for ASCL2 Gene

PMID Author Year ID in Paper
 Conclusion
30106147 Qingsong Zuo 2018 ASCL2 The results of the present study demonstrated that ASCL2 was able to downregulate the expression level of miR223, contribute to EMT and promote gastric tumor metastasis, which indicated that ASCL2 may serve as a therapeutic target in the treatment of GC.
29746925 Tao Wang 2018 ASCL2 a Wnt-responsive CSC pathway that could be exploited to identify essential regulators of the signaling output, and reveal SMYD3 as an epigenetic target for eliminating CSCs in human cancers.

Experiment for ASCL2 Gene

Sample Type Sample Number GC Sample Number nonGC Method Region Hpylori Normal vs. GC Early-stage vs. Advanced stage Biological Functions in GC Cells Result PMID
tissue 32 32 qRT-PCR:Western blot; IHC China No Reports Up - - Expression of ASCL2 is highest in metastases, among adjacent normal tissues, primary gastric tumors and gastric metastases. 30106147
cell line - - Cell invasion and migration assays - No Reports - - ASCL2 expression contributes to cell migration and invasion in GC cells. ASCL2 expression contributes to cell migration and invasion in GC cells. 30106147
cell line - - Western blot - No Reports - - - the expression of E-cadherin in ASCL2-overexpressing cell lines decreased, while Zeb-1, twist-related protein 1 (Twist), Integrin, Vimentin, 72 kDa type IV collagenase (MMP-2) and matrix metalloproteinase 9 (MMP-9) were upregulated in ASCL2-overexpressing cell lines 30106147
cell line - - Luciferase reporter assay; ChIP - No Reports - - - ASCL2 may interact with the promoter of pre-miR223, and inhibit the maturation of miR223. 30106147
tissue 24 24 qRT-PCR China No Reports Up - - The mRNA levels of ASCL2 were significantly increased in GC tissues compared to ad- jacent non-tumor gastric epithelium; ASCL2 expression in GC correlated with poor histological differentiation, tumor cell invasion into lymph nodes, and higher TNM stages (III + IV). SMYD3 expression status correlates with ASCL2 in GC tissues. 29746925
tissue - - IHC China No Reports - - - ASCL2 protein were detected in the nuclei of GC cells 29746925
cell line - - qRT-PCR; FACS; Tumorsphere formation assay - No Reports - - - ASCL2+ cells showed increased propensity of growing as tumorspheres 29746925
mice - - Xenograft mice models - No Reports - - - High levels of ASCL2 expression are necessary for stemness and tumorigenicity of ASCL2+ GC cells 29746925
cell line - - ChIP-qPCR - No Reports - - - SMYD3 maintains high H3K4me3 levels on the ASCL2 promoter in ASCL2+ cells, indicating its potential role in regulating ASCL2 transcription and expression. 29746925
cell line - - qRT-PCR; Western blot - No Reports - - - Depletion of SMYD3 inhibits Wnt-induced upregulation of ASCL2. 29746925

Function for ASCL2 Gene

Functional Type AUC Overall Survival Disease-free Survival
target - - -
target/prognosis - < 0.001 -

Interaction for ASCL2 Gene

Interaction Gene Method PMID
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