| Sample Type |
Sample Number GC |
Sample Number nonGC |
Method |
Region |
Hpylori |
Normal vs. GC |
Early-stage vs. Advanced stage |
Biological Functions in GC Cells |
Result |
PMID |
| tissue |
236 |
236 |
CHIP assay |
China |
No Reports |
- |
- |
- |
DNA copy-number of C8orf76 was frequently gained in gastric cancer. |
30733230 |
| tissue |
146 |
146 |
qRT-PCR |
China |
No Reports |
Up |
- |
- |
C8orf76 was upregulated in 69.74% and 65.71% of two independent cohorts of gastric cancers and was positively associated with C8orf76 amplification. Multivariate analysis showed that gastric cancer patients with C8orf76 amplification (cohort I, n = 129; cohort II, n = 107) or overexpression (n = 356) had a significantly shortened survival. C8orf76 significantly promoted gastric cancer cell proliferation, cell-cycle transformation, and migration/invasion, but suppressed cell apoptosis. Silencing C8orf76 expression exerted opposite effects in vitro and significantly inhibited xenograft tumor growth, lung metastasis, and liver metastasis in nude mice. Silencing C8orf76 also significantly suppressed the growth of patient-derived organoids. Mechanically, C8orf76 activated MAPK/ERK signaling cascade. C8orf76 directly bound to the promoter region of lncRNA dual specificity phosphatase 5 pseudogene 1 (DUSP5P1) with a binding motif of AGGCTG and activated DUSP5P1 transcription. DUSP5P1 induced MAPK/ERK signaling and promoted gastric tumorigenesis. Knockdown DUSP5P1 abrogated the effect of C8orf76 in activating MAPK/ERK cascade and the tumor-promoting function. |
30733230 |
| tissue |
146 |
146 |
IHC |
China |
No Reports |
Up |
- |
- |
C8orf76 protein expression was significantly higher in primary gastric tumors as compared with adjacent nontumor tissues. |
30733230 |
| tissue |
356 |
- |
IHC |
China |
No Reports |
- |
- |
- |
High C8orf76 protein expression was significantly associated with more advanced tumor stage and diffuse-type gastric cancer. C8orf76 is a poor prognostic factor in patients with gastric cancer. |
30733230 |
| cell line |
- |
- |
Cell proliferation assay; Invasion and migration assays; Flow cytometry |
- |
No Reports |
- |
- |
C8orf76 promotes cell growth and cell-cycle progression, but inhibits cell apoptosis |
C8orf76 promotes cell growth and cell-cycle progression, but inhibits cell apoptosis |
30733230 |
| mice |
- |
- |
Xenograft mice models |
- |
No Reports |
- |
- |
- |
C8orf76 promotes the growth of subcutaneous xenograft tumors in nude mice; C8orf76 promotes gastric cancer metastasis to lung and liver in vivo. |
30733230 |
| tissue |
- |
- |
Patient-derived gastric cancer organoid model |
- |
No Reports |
- |
- |
- |
Knockdown of C8orf76 significantly suppressed the gastric cancer cell growth in three PDO models |
30733230 |
| cell line |
- |
- |
Luciferase reporter assay; Western blot; qRT-PCR; ChIP-PCR |
- |
No Reports |
- |
- |
- |
DUSP5P1 is a direct downstream target of C8orf76 |
30733230 |
