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Gene Summary for MIR203A Gene

Official Symbol MIR203A Gene Type miRNA
Full Name microRNA 203a Entrez 406986
Ensembl ID ENSG00000207568 HGNC 31581
Aliases MIR203, MIRN203, hsa-mir-203a, miR-203, miRNA203, mir-203a Chromosome Location Chromosome 14, NC_000014.9 (104117405..104117514)
Expression -
Summary microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]

Paper for MIR203A Gene

PMID Author Year ID in Paper
 Conclusion
30837034 Jianye Li 2019 miR-203 reactivation of the miR-203/annexin A4 axis may represent a new strategy for overcoming metastasis of GC.
25373785 Xiaoying Zhou 2014 miR-203 miR-203 functions as a growth-suppressive miRNA in H. pylori related GC, and that its suppressive effects are mediated mainly by repressing CASK expression.

Experiment for MIR203A Gene

Sample Type Sample Number GC Sample Number nonGC Method Region Hpylori Normal vs. GC Early-stage vs. Advanced stage Biological Functions in GC Cells Result PMID
tissue 10 10 qRT-PCR China No Reports Down - - miR-203 is downregulated in GC tissues and cell lines. Moreover, the low level of miR-203 was associated with increased expression of annexin A4 in GC tissues and cell lines. 30837034
cell line - - Cell invasion and migration assays - No Reports - - miR-203 Suppressed the Invasion and EMT of GC Cells Through Inhibition of Annexin A4 miR-203 Suppressed the Invasion and EMT of GC Cells Through Inhibition of Annexin A4 30837034
cell line - - Luciferase reporter assay - No Reports - - - miR-203 Directly Targets Annexin A4 in GC Cells 30837034
cell line - - qRT-PCR; Western blot - No Reports - - - overexpression of miR-203 could inhibit EMT of GC cells 30837034
tissue 50 50 qRT-PCR China Related Down - - miR-203 is significantly down-regulated in H. pylori positive tissues and cells and in tumor tissues with important functional consequences. 25373785
cell line - - Cell proliferation assay; Invasion and migration assays; Clonal formation assay - No Reports - - Ectopic expression of miR-203 dramatically suppressed cell proliferation and invasion. We found that miR-203 strongly reduced the expression of CASK oncogene in GC cells. Similar to the restoring miR-203 expression, CASK down-regulation inhibited cell growth and invasion, whereas CASK over-expression rescued the suppressive effect of miR-203. These results can also be found in nude mice. In clinical specimens, CASK was over-expressed in tumors and H. pylori positive tissues and its mRNA levels were inversely correlated with miR-203 expression Ectopic expression of miR-203 dramatically suppressed cell proliferation and invasion. We found that miR-203 strongly reduced the expression of CASK oncogene in GC cells. Similar to the restoring miR-203 expression, CASK down-regulation inhibited cell growth and invasion, whereas CASK over-expression rescued the suppressive effect of miR-203. These results can also be found in nude mice. In clinical specimens, CASK was over-expressed in tumors and H. pylori positive tissues and its mRNA levels were inversely correlated with miR-203 expression 25373785
cell line - - Luciferase reporter assay - No Reports - - - CASK was a direct target of miR-203 in GC cells. 25373785
mice - - Xenograft mice models - No Reports - - - MiR-203 suppressed tumor growth of GC cells in nude mice. 25373785

Function for MIR203A Gene

Functional Type AUC Overall Survival Disease-free Survival
target - - -
mechanism - - -

Interaction for MIR203A Gene

Interaction Gene Method PMID
ANXA4 Luciferase reporter assay 30837034
CASK Luciferase reporter assay 25373785
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