MKGC: a curated molecular knowledgebase for gastric cancer


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Gene Summary for MIR302B Gene

Official Symbol MIR302B Gene Type miRNA
Full Name microRNA 302b Entrez 442894
Ensembl ID ENSG00000284463 HGNC 31763
Aliases MIRN302B; mir-302b Chromosome Location Chromosome 14, NC_000004.12 (112648485..112648557, complement)
Expression
Summary microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]

Paper for MIR302B Gene

PMID Author Year ID in Paper
Conclusion
28743112 Bo Guo 2017 miR-302b-3p These results indicate the tumor suppressor role of miR-302b-3p in the pathogenesis of gastric cancer.

Experiment for MIR302B Gene

Sample Type Sample Number GC Sample Number nonGC Method Region Hpylori Normal vs. GC Early-stage vs. Advanced stage Biological Functions in GC Cells Result PMID
tissue 20 20 qRT-PCR China No Reports Down - - MiR-302b-3p expression was decreased in gastric cancer tissues and cell lines. 28743112
cell line - - Cell proliferation assay; Cell colony formation assay; Apoptosis assay; Flow cytometry - No Reports - - Enforced expression of miR-302b suppressed cell proliferation and cell cycle G1-S transition and induced apoptosis. Enforced expression of miR-302b suppressed cell proliferation and cell cycle G1-S transition and induced apoptosis. 28743112
cell line - - Luciferase reporter assay - No Reports - - - IGF-1R was found to be a direct target of miR-302b-3p, and silencing of IGF-1R resulted in the same biological effects as those induced by miR-302b-3p overexpression in gastric cancer cells. Both overexpression of miR-302b-3p and silencing of IGF-1R decreased AKT phosphorylation, which modulated AKT related cell cycle regulators (cyclin A2, cyclin D1, CDK2, and CDk6) and apoptotic protein Bax/Bcl-2. 28743112

Function for MIR302B Gene

Functional Type AUC Overall Survival Disease-free Survival
mechanism - - -

Interaction for MIR302B Gene

Interaction Gene Method PMID
IGF-1R Luciferase reporter assay 28743112
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