Gastric Cancer Database

Official Symbol	MIR320A	Gene Type	miRNA
Full Name	microRNA 320a	Entrez	407037
Ensembl ID	ENSG00000208037	HGNC	31632
Aliases	MIRN320, MIRN320A, hsa-mir-320a, mir-320a	Chromosome Location	Chromosome 8, NC_000008.11 (22244966..22245037, complement)
Expression	-
Summary	microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]

PMID	Author	Year	ID in Paper	Conclusion
29152656	Xiancai Ge	2017	miR-320a	miR-320a may function as a tumor suppressor in GC progression and potential therapeutic strategies for GC may be based on the miR-320a/ADAM10 axis.
27086911	Yangyang Wang	2016	MiR-320a	These results thus reveal the crucial role played by miR-320a in limiting gastric carcinoma by directly targeting FoxM1- P27KIP1 axis.

Sample Type	Sample Number GC	Sample Number nonGC	Method	Region	Hpylori	Normal vs. GC	Early-stage vs. Advanced stage	Biological Functions in GC Cells	Result	PMID
tissue	40	40	qRT-PCR	China	No Reports	Down	-	-	Downregulated in GC tumor tissues; miR‑320a is negatively correlated with ADAM10 in tumors	29152656
cell line	-	-	Colony formation assay; MTS assay; Viability assay	-	No Reports	-	-	-	miR‑320a inhibits cell growth and enhances sensitivity of GC cells to cisplatin and suppresses tumor growth in vivo.	29152656
cell line	-	-	Luciferase reporter assay	-	No Reports	-	-	-	miR‑320a directly targets ADAM10 in GC cells.	29152656
tissue	14	14	qRT-PCR	China	No Reports	Down	-	-	miR-320a expression is decreased in human gastric cancer tissues and correlates inversely with expression of FoxM1, a key cell cycle regulator involved in gastric carcinoma. By contrast, the expression of P27KIP1, a downstream effector of FoxM1, correlates positively with miR-320a levels.	27086911
cell line	-	-	Clone formation assay	-	No Reports	-	-	miR-320a inhibits gastric cancer cell proliferation by suppressing activity in the FoxM1-P27KIP1 axis.	miR-320a inhibits gastric cancer cell proliferation by suppressing activity in the FoxM1-P27KIP1 axis.	27086911
mice	-	-	Xenograft mice models	-	No Reports	-	-	-	nude mice injected with BGC-823 gastric cancer cells expressing a miR-320a inhibitor exhibit faster tumor growth than mice injected with control cells.	27086911
cell line	-	-	Luciferase reporter assay	-	No Reports	-	-	-	FoxM1 is a direct target of miR-320a	27086911