Search Result
Gene Summary for MIR874 Gene
| Official Symbol |
MIR874 |
Gene Type |
miRNA |
| Full Name |
microRNA 874 |
Entrez |
100126343
|
| Ensembl ID |
ENSG00000216009 |
HGNC |
33643
|
| Aliases |
MIRN874, hsa-mir-874, mir-874
|
Chromosome Location |
Chromosome 5, NC_000005.10 (137647572..137647649, complement)
|
| Expression |
-
|
| Summary |
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]
|
Paper for MIR874 Gene
| PMID |
Author |
Year |
ID in Paper
|
Conclusion |
| 23800944 |
Baofei Jiang |
2014 |
miR-874 |
AQP3 is upregulated, as well as highlight the importance of miR-874 in gastric cancer development and progression. |
Experiment for MIR874 Gene
| Sample Type |
Sample Number GC |
Sample Number nonGC |
Method |
Region |
Hpylori |
Normal vs. GC |
Early-stage vs. Advanced stage |
Biological Functions in GC Cells |
Result |
PMID |
| tissue |
75 |
75 |
qRT-PCR |
China |
No Reports |
Down |
Down |
- |
miR-874 was significantly down-regulated and reversely correlated with AQP3 protein levels in clinical samples. miR-874 expression level was lower in samples with moderately and poorly histological type, lymph node metastasis N1-N3 and stage III–IV. Analysis of the clinicopathological significance showed that miR-874 and AQP3 were closely correlated with GC characteristics. |
23800944 |
| cell line |
- |
- |
Cell proliferation assay; Invasion and migration assays; Flow Cytometry |
- |
No Reports |
- |
- |
Functional analyses indicated that ectopic miR-874 expression suppressed the growth, migration, invasion and tumorigenicity of GC cells, whereas miR-874 knockdown promoted these phenotypes. Down-regulation of Bcl-2, MT1-MMP, MMP-2 and MMP-9 and upregulation of caspase-3 activity and Bax were involved in miR-874 inducing cell apoptosis, and inhibiting migration and invasion. |
Functional analyses indicated that ectopic miR-874 expression suppressed the growth, migration, invasion and tumorigenicity of GC cells, whereas miR-874 knockdown promoted these phenotypes. Down-regulation of Bcl-2, MT1-MMP, MMP-2 and MMP-9 and upregulation of caspase-3 activity and Bax were involved in miR-874 inducing cell apoptosis, and inhibiting migration and invasion. |
23800944 |
| mice |
- |
- |
Xenograft mice models |
- |
No Reports |
- |
- |
- |
miR-874 suppresses the tumorigenicity in vivo |
23800944 |
| cell line |
- |
- |
Luciferase reporter assay |
- |
No Reports |
- |
- |
- |
miR-874 directly interacts with a putative binding site of AQP3-3'UTR |
23800944 |
Function for MIR874 Gene
| Functional Type |
AUC |
Overall Survival |
Disease-free Survival |
| mechanism |
- |
- |
- |
Interaction for MIR874 Gene
| Interaction Gene |
Method |
PMID |
|
AQP3 |
Luciferase reporter assay |
23800944
|
|
