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Gene Summary for MIRLET7B Gene

Official Symbol MIRLET7B Gene Type miRNA
Full Name microRNA let-7b Entrez 406884
Ensembl ID ENSG00000284520 HGNC 31479
Aliases LET7B, MIRNLET7B, hsa-let-7b, let-7b Chromosome Location Chromosome 22, NC_000022.11 (46113686..46113768)
Expression -
Summary microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]

Paper for MIRLET7B Gene

PMID Author Year ID in Paper
 Conclusion
30237418 Xiao Han 2018 Let-7b Overexpression of let-7b might provide a potential strategic approach for attenuating DDP resistance in cisplatin_x005fresistant human gastric cancer cell line (SGC7901/DDP).
25510669 Junbo Yu 2015 Let-7b let-7b may directly target Cthrc1 and function as a tumor suppressor gene in GC.
25288334 Wei Kang 2014 let-7b Decreased let-7b/g contributes to aberrant AKT activation in gastric tumorigenesis and provide a potential therapeutic strategy for gastric cancer.

Experiment for MIRLET7B Gene

Sample Type Sample Number GC Sample Number nonGC Method Region Hpylori Normal vs. GC Early-stage vs. Advanced stage Biological Functions in GC Cells Result PMID
cell line - - qRT-PCR - No Reports - - - let-7b-expression level of SGC7901/DDP cells was significantly lower than for its parental SGC7901 cells. 30237418
cell line - - Cell proliferation assay; Flow cytometry; - No Reports - - Upregulation of let-7b could reverse the DDP resistance of gastric cancer SGC7901/DDP cells by inducing cell viability inhibition and apoptosis when exposed to DDP treatment. Upregulation of let-7b could reverse the DDP resistance of gastric cancer SGC7901/DDP cells by inducing cell viability inhibition and apoptosis when exposed to DDP treatment. 30237418
cell line - - Luciferase reporter assay - No Reports - - - let-7b directly targets AURKB by binding to its 3'UTR region in DDP-resistant gastric cancer cells to DDP. 30237418
mice - - Xenograft mice models - No Reports - - - Upregulation of let-7b inhibited tumor growth derived from DDP-resistant cells in vivo. 30237418
tissue 47 47 qRT-PCR China Related Down - - Let-7b was found downregulated remarkably in gastric cancer tissues and was correlated with Helicobacter pylori infection, tumor stage, and lymphatic metastasis. 25510669
cell line - - Cell proliferation assay; Cell invasion and migration assays; Cell colony formation assay - No Reports - - Ectopic expression of let-7b suppressed the growth, migration, invasion, and tumorigenicity of GC cells, whereas let-7b knockdown promoted these phenotypes. Ectopic expression of let-7b suppressed the growth, migration, invasion, and tumorigenicity of GC cells, whereas let-7b knockdown promoted these phenotypes. 25510669
mice - - Xenograft mice models - No Reports - - - Let-7b suppresses the tumorigenicity in vivo. 25510669
cell line - - Luciferase reporter assay - No Reports - - - Cthrc1 was a direct target of let-7b. 25510669
tissue 76 76 qRT-PCR China No Reports Down - - let-7b was down-regulated in gastric cancer and its downregulation was associated with poor survival and correlated with lymph node metastasis. AKT2 mRNA expression showed negative correlation with the expression of let-7b in primary tumors. 25288334
cell line - - Cell proliferation assay; Invasion assay; Cell colony fomation assay - No Reports - - suppressed the growth and invasive capacityof GC cells suppressed the growth and invasive capacityof GC cells 25288334
mice - - Xenograft mouse models - No Reports - - let-7b/g transfectants formed smaller xenografts than those scramble transfectants let-7b/g transfectants formed smaller xenografts than those scramble transfectants 25288334
cell line - - Luciferase reporter assay - No Reports - - - let-7b/g inhibited AKT2 expression by directly binding to its 3'UTR, reduced p-AKT (S473) activation and suppressed expression of the downstream effector pS6. 25288334

Function for MIRLET7B Gene

Functional Type AUC Overall Survival Disease-free Survival
target - - -
target/prognosis - - 0.001

Interaction for MIRLET7B Gene

Interaction Gene Method PMID
AURKB Luciferase reporter assay 30237418
CTHRC1 Luciferase reporter assay 25510669
AKT2 Luciferase reporter assay 25288334
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